Obtainment and characterization of digestive aspartic proteases from the fish Caranx hippos (Linnaeus, 1766)

نویسندگان

چکیده

Abstract This work aimed to obtain aspartic proteases of industrial and biotechnological interest from the stomach crevalle jack fish (Caranx hippos). In order do so, a crude extract (CE) was obtained subjected partial purification by salting-out, which resulted in enzyme (EE) obtainment. EE were characterized physicochemically means zymogram. addition, effect chemical agents on their activity also assessed. By salting-out it possible 1.6 times with yield 49.4%. Two acid present observed The optimum temperature thermal stability for acidic 55 ºC 45 °C, respectively. pH found these enzymes 1.5 7.0, Total inhibition proteolytic presence pepstatin A. dithiothreitol (DTT) Ca2+ did not promote significant activity. heavy metals, such as Al3+, Cd2+ Hg2+, showed more than 70% enzymatic results show that is obtain, C. hippos, high characteristics demonstrate potential applications.

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ژورنال

عنوان ژورنال: Brazilian Journal of Biology

سال: 2022

ISSN: ['1519-6984', '1678-4375']

DOI: https://doi.org/10.1590/1519-6984.234500